This is my PCR product gel image, post post for "shuang"... Hehe...
Figure 1: PCR Products (100bp ladder, Ami-203, C391, Cj18, CUD-68, Cuj131, -ve)
Figure 2: PCR Products (100bp ladder, -ve, Ami-203, C391, Cj18, CUD-68, Cuj131)
Discussion:
- Thinner gel better, coz you can see more bands for Figure 2 (which is thinner gel)
- Smaller EZ Vision volume (loading + staining solution) is better, coz less dark area for Figure 2
- 3% gel not easy to cast, coz there is some "condong" bands at lane 6 and 7 in Figure 2, which is caused by uneven gel solidification
- FYP not finish yet... :) + :(
P/S: Hope to see other interesting results... Hehe...
