See what I have Blasted...

Just now, I helped my friend to blast her ~500 bp sequence of 16s rRNA gene of an unknown bacteria collected from soil.

Guess what is the result? [click to enlarge]

The E-value is low (in fact, it is so low it is zero). Low E value means it is highly possible that particular organism is the one you are looking for. In this case, it is Bacillus anthracis. OH... MY... GOD.................

Although it only says a partial sequence, a 500 bp out of the total 1400 bp 16S rRNA gene and with a score of 900+ and E value = 0, it has a good chance to be right! Then and again, a full-length gene is needed for a 100% identity. Sigh, too bad it is not a full length one.